Application of DNA-free CRISPR/Cas-mediated Genome Editing in Crops: A Review

Clustered regularly interspaced short palindromic repeats/CRISPR associated nuclease 9 (CRISPR-Cas9) system was instigated first into eukaryotes in the last ten years are becoming productive and worldwide application for genome modification. Genome engineering through insertion of foreign DNA insert have numerous disadvantages which could be overcome by use DNA-free editing. Various ways editing mediated CRISPR/Cas systems delivery as ribonucleoprotein, virus-like particles agrobacterium-based CRISPR/Cas. Crop improvement via been applied rice, wheat, maize, tomato, soybean rare species like Nicotiana benthamiana etc. It is method choice precise without shuffle an organism.